6MJO

CRYSTAL STRUCTURE OF RHESUS MACAQUE (MACACA MULATTA) FC-GAMMA RECEPTOR III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	49.073, 63.960, 69.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.989 - 1.900
R-factor	0.1838
Rwork	0.182
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e4j
RMSD bond length	0.015
RMSD bond angle	1.636
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.153	0.704
Rpim	0.067	0.435
Number of reflections	17804	836
<I/σ(I)>	27.4	1.4
Completeness [%]	99.2	96
Redundancy	5.9	4.7
CC(1/2)	0.990	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	25% PEG 3350 0.1M HEPES pH 7.0