6MJE
Structure of Candida glabrata Csm1: S. cerevisiae Dsn1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-15 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.127 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.368, 203.755, 44.259 |
Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
Resolution | 43.351 - 2.500 |
R-factor | 0.2426 |
Rwork | 0.240 |
R-free | 0.29720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n4r |
RMSD bond length | 0.007 |
RMSD bond angle | 0.891 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.199 | 0.793 |
Rmeas | 0.219 | 0.938 |
Rpim | 0.091 | 0.488 |
Number of reflections | 26122 | 1446 |
<I/σ(I)> | 18.2 | |
Completeness [%] | 96.0 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, 25% PEG3350, cryoprotect with additional 20% PEG400 |