6MJ8
Structure of Candida glabrata Csm1:Mam1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.124, 58.639, 109.831 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.915 - 3.030 |
R-factor | 0.272 |
Rwork | 0.271 |
R-free | 0.28290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n4r |
RMSD bond length | 0.005 |
RMSD bond angle | 0.763 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 3.200 |
High resolution limit [Å] | 3.030 | 3.030 |
Rmerge | 0.308 | 1.651 |
Rmeas | 0.358 | 1.919 |
Rpim | 0.136 | 0.714 |
Number of reflections | 6723 | 958 |
<I/σ(I)> | 8.4 | |
Completeness [%] | 100.0 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES, pH 6.5, 0.6 M sodium chloride, 20% PEG4000, cryoprotectant: 20% PEG400 |