6MJ7
Crystal structure of p62 ZZ domain in complex with free arginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-10-12 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.278 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 44.971, 32.269, 34.941 |
Unit cell angles | 90.00, 122.16, 90.00 |
Refinement procedure
Resolution | 29.579 - 1.412 |
R-factor | 0.1386 |
Rwork | 0.136 |
R-free | 0.16460 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.811 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2567)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.430 |
High resolution limit [Å] | 1.410 | 1.410 |
Rmerge | 0.063 | |
Number of reflections | 107631 | |
<I/σ(I)> | 83.2 | |
Completeness [%] | 98.4 | |
Redundancy | 13.2 | |
CC(1/2) | 0.985 | 0.985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Bicine (pH 9.0), 14% PEG 20K, 4% 1,4-Dioxane |