6MJ3

CRYSTAL STRUCTURE OF RHESUS MACAQUE (MACACA MULATTA) IGG1 Fc Fragment-Fc-GAMMA RECEPTOR III complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	213.108, 71.270, 135.521
Unit cell angles	90.00, 119.72, 90.00

Refinement procedure

Resolution	66.510 - 3.800
R-factor	0.2782
Rwork	0.276
R-free	0.32360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e4k
RMSD bond length	0.006
RMSD bond angle	1.148
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.510	4.010
High resolution limit [Å]	3.800	3.800
Rpim	0.145	0.496
Number of reflections	14678	2222
<I/σ(I)>	3.4	1
Completeness [%]	83.9	86.9
Redundancy	2.3	2.3
CC(1/2)	0.960	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	15% PEG 4000, 0.1M HEPES pH 7.0, 0.1M magnesium chloride