6MJ1
Crystal structure of RefZ (regulator of FtsZ) from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2014-07-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 100.021, 100.021, 100.177 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.745 - 2.600 |
R-factor | 0.2236 |
Rwork | 0.222 |
R-free | 0.25360 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.082 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | SHELXDE |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.113 | 0.793 |
Number of reflections | 16237 | 714 |
<I/σ(I)> | 11.59 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 17.59 | 14.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 289 | 10% ethanol (v/v), 0.1 M imidazole [pH 8.0], and 0.2 M MgCl2 |