6MIW
WWE domain of human HUWE1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-08-23 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.134, 60.134, 53.288 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.090 - 2.000 |
| R-factor | 0.1814 |
| Rwork | 0.180 |
| R-free | 0.21180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FFAS model based on PDB entry 1ujr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.715 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.070 | 30.070 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.093 | 0.023 | 1.073 |
| Rmeas | 0.098 | 0.024 | 1.130 |
| Rpim | 0.030 | 0.008 | 0.352 |
| Total number of observations | 81684 | 875 | 5967 |
| Number of reflections | 7843 | ||
| <I/σ(I)> | 22.4 | 81.6 | 2.4 |
| Completeness [%] | 100.0 | 97.9 | 100 |
| Redundancy | 10.4 | 8.3 | 10.3 |
| CC(1/2) | 0.999 | 1.000 | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Molecular Dimensions Morpheus HT condition D3 |
