6MIU

Crystal structure of p62 ZZ domain in complex with Arg-Glu peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-23
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	26.990, 27.864, 38.994
Unit cell angles	90.06, 90.45, 110.30

Refinement procedure

Resolution	25.313 - 1.900
R-factor	0.1606
Rwork	0.155
R-free	0.21230
RMSD bond length	0.007
RMSD bond angle	1.080
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.313	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.064
Number of reflections	51427
<I/σ(I)>	27.8
Completeness [%]	99.5	93.1
Redundancy	6.1
CC(1/2)	0.955	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M BICINE, pH 8.0, 20% PEG 6000