6MIQ

Crystal structure of Taf14 YEATS domain in complex with histone H3K9bu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-06-19
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	113.149, 113.149, 26.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.575 - 1.750
R-factor	0.1745
Rwork	0.172
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iok
RMSD bond length	0.006
RMSD bond angle	0.909
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.000	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	31249
<I/σ(I)>	34
Completeness [%]	99.9
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	44% PEG600 (v/v) and 0.2 M citric acid (pH 6.0)