6MIP

Crystal structure of Taf14 YEATS domain G82A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-22
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	113.364, 113.364, 26.222
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.107 - 2.000
R-factor	0.1819
Rwork	0.180
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iok
RMSD bond length	0.007
RMSD bond angle	0.882
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	17536
<I/σ(I)>	35.9
Completeness [%]	92.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	48% PEG600 with 0.2 M sodium citrate (pH 6.0)