6MIJ
Crystal structure of EF-Tu from Acinetobacter baumannii in complex with Mg2+ and GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97894 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.386, 71.578, 114.002 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.129 - 1.955 |
R-factor | 0.1926 |
Rwork | 0.190 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zv4 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.798 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_3092: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.070 | 0.635 |
Rpim | 0.030 | 0.267 |
Number of reflections | 34238 | 1676 |
<I/σ(I)> | 33.81 | 2.46 |
Completeness [%] | 99.0 | 97.7 |
Redundancy | 6.4 | 6.3 |
CC(1/2) | 0.918 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M magnesium format, 20% PEG3350, cryoprotectant paratone |