6MIJ

Crystal structure of EF-Tu from Acinetobacter baumannii in complex with Mg2+ and GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97894
Spacegroup name	P 21 21 21
Unit cell lengths	57.386, 71.578, 114.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.129 - 1.955
R-factor	0.1926
Rwork	0.190
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zv4
RMSD bond length	0.006
RMSD bond angle	0.798
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_3092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.070	0.635
Rpim	0.030	0.267
Number of reflections	34238	1676
<I/σ(I)>	33.81	2.46
Completeness [%]	99.0	97.7
Redundancy	6.4	6.3
CC(1/2)		0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M magnesium format, 20% PEG3350, cryoprotectant paratone