6MIC
Crystal Structure of the C-terminal half of the Vibrio cholerae minor pilin TcpB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 63.290, 63.290, 236.291 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.382 - 1.531 |
R-factor | 0.1794 |
Rwork | 0.178 |
R-free | 0.19820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qs4 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.991 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.800 | 1.560 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.028 | 0.091 |
Rmeas | 0.033 | 0.105 |
Rpim | 0.016 | 0.053 |
Number of reflections | 26889 | 1366 |
<I/σ(I)> | 26.9 | 12.4 |
Completeness [%] | 96.1 | 99.4 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.999 | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | MPD, sodium chloride, sodium acetate |