6MI3

Structure of NEMO(51-112) with N- and C-terminal coiled-coil adaptors.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919774
Spacegroup name	P 1 21 1
Unit cell lengths	64.480, 33.620, 76.870
Unit cell angles	90.00, 115.04, 90.00

Refinement procedure

Resolution	37.754 - 1.783
R-factor	0.2441
Rwork	0.243
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dmd
RMSD bond length	0.012
RMSD bond angle	1.141
Data reduction software	XDS
Data scaling software	STARANISO (v3.000)
Phasing software	PHASER
Refinement software	PHENIX (dev_2849)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.754	1.847
High resolution limit [Å]	1.783	1.783
Rmerge	0.080	5.483
Rmeas	0.096	6.493
Rpim	0.052	3.444
Number of reflections	28586	431
<I/σ(I)>	5.71	0.24
Completeness [%]	67.5	15.12
Redundancy	3.3	3.4
CC(1/2)	0.997	0.089

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.1 M Tris pH 8.0, 5.8% PGA-LM, 5.45% PEG 20,000