6MHT
TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND DNA CONTAINING 4'-THIO-2'DEOXYCYTIDINE AT THE TARGET
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 99.860, 99.860, 325.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 5.000 - 2.050 |
R-factor | 0.186 * |
Rwork | 0.186 |
R-free | 0.23100 |
Structure solution method | OTHER |
Starting model (for MR) | 5mht |
RMSD bond length | 0.010 |
RMSD bond angle | 25.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.050 |
Rmerge | 0.078 |
Number of reflections | 32880 |
<I/σ(I)> | 12.1 |
Completeness [%] | 98.0 |
Redundancy | 3.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.6 | 16 * | O'Gara, M., (1996) J. Mol. Biol., 261, 634. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 1 | citrate | ||
3 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | protein | 10 (mg/ml) | ||
2 | 1 | ammonium sulfate | 1.4-1.8 (M) | ||
3 | 1 | 1 | citrate | 50 (mM) | |
4 | 1 | 2 | ammonium sulfate | 1.4-1.8 (M) | |
5 | 1 | 2 | citrate | 50 (mM) |