6MHN
Photoactive Yellow Protein with covalently bound 3-chloro-4-hydroxycinnamic acid chromophore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-09-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.250, 66.250, 40.661 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.175 - 1.670 |
| R-factor | 0.1724 |
| Rwork | 0.170 |
| R-free | 0.21470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nwz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.370 | 1.710 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.124 | |
| Number of reflections | 17696 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 21.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1M sodium chloride, 2.4M ammonium sulfate, pH = 6.0, drops were microseeded |
