6MHH
Proteus mirabilis ScsC linker (residues 39-49) deletion and N6K mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-06 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.541870 |
Spacegroup name | H 3 2 |
Unit cell lengths | 64.040, 64.040, 299.834 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.986 - 2.083 |
R-factor | 0.1759 |
Rwork | 0.174 |
R-free | 0.20960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4XVW (chain A residues 47-224) |
RMSD bond length | 0.003 |
RMSD bond angle | 0.687 |
Data reduction software | iMOSFLM (7.1.3) |
Data scaling software | Aimless (0.5.8) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.990 | 24.990 | 2.140 |
High resolution limit [Å] | 2.080 | 9.080 | 2.080 |
Rmerge | 0.059 | 0.061 | 0.140 |
Rmeas | 0.063 | 0.065 | 0.150 |
Rpim | 0.020 | 0.022 | 0.053 |
Number of reflections | 14715 | 207 | 1135 |
<I/σ(I)> | 27.2 | ||
Completeness [%] | 99.9 | 94.8 | 100 |
Redundancy | 10.1 | 8.8 | 7.8 |
CC(1/2) | 0.998 | 0.996 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7, 32% v/v Jeffamine M-600 |