6MH4
Crystal Structure of 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Staphylococcus schleiferi, Apoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 71.368, 71.368, 318.815 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.380 - 2.150 |
R-factor | 0.1991 |
Rwork | 0.197 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.061 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.380 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 50897 | 5069 |
<I/σ(I)> | 33.9 | |
Completeness [%] | 99.2 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 2000 mM ammonium sulfate, 100 mM sodium citrate/ citric acid |