6MGD

Thermosulfurimonas dismutans KpsC, beta Kdo 2,7 transferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-24
Detector	RAYONIX MX300HE
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	55.670, 79.820, 77.400
Unit cell angles	90.00, 109.12, 90.00

Refinement procedure

Resolution	37.299 - 2.500
R-factor	0.2304
Rwork	0.229
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.550
High resolution limit [Å]	2.500	2.500
Number of reflections	22210
<I/σ(I)>	15.5	2.2
Completeness [%]	99.8	100
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M HEPES, 10% v/v PEG6000, 5 mg/mL protein with 1 mM CMP, 50 mM L-Arg