6MGC

Escherichia coli KpsC, N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	58.220, 64.580, 77.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.568 - 1.350
R-factor	0.1563
Rwork	0.155
R-free	0.17380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.979
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.390
High resolution limit [Å]	1.350	1.350
Rmerge	0.063	0.710
Rpim	0.027	0.330
Number of reflections	64850
<I/σ(I)>	14.7	2.15
Completeness [%]	99.9	99.3
Redundancy	6.4	5.5
CC(1/2)	0.997	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.25	293	0.2 M lithium sulfate, 0.1 M Bis-Tris, 19% v/v PEG3350, 15 mg/mL protein with 1 mM CMP