6MGA

Crystal Structure of Human Protocadherin-1 EC1-4 with glycosylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 61 2 2
Unit cell lengths	147.227, 147.227, 149.366
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.530 - 3.150
R-factor	0.21715
Rwork	0.215
R-free	0.26342
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bx7
RMSD bond length	0.009
RMSD bond angle	1.503
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.150	3.150
Rmerge	0.078	1.762
Rmeas	0.079	1.792
Rpim	0.013	0.321
Number of reflections	17231
<I/σ(I)>	58	2.5
Completeness [%]	100.0	99.9
Redundancy	36.4	28.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1 M Magnesium Chloride, 0.05 M Tris HCl pH 8.5, 30% PEG 550 MME