6MG2

C-terminal bZIP domain of human C/EBPbeta with 16bp Methylated Oligonucleotide Containing Consensus Recognition Sequence-C2221 Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-03
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	101.854, 113.648, 75.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.903 - 1.928
R-factor	0.1769
Rwork	0.175
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e42
RMSD bond length	0.005
RMSD bond angle	0.939
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.903	2.000
High resolution limit [Å]	1.928	1.928
Rmerge	0.081
Rpim	0.020	0.287
Number of reflections	63673	6293
<I/σ(I)>	31.2	2.7
Completeness [%]	99.8	99.6
Redundancy	16.6	12.8
CC(1/2)		0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.1 M sodium acetate trihydrate pH 7.0, 12% (w/v) polyethylene glycol 3350