6MFK
Crystal Structure of Chloramphenicol Acetyltransferase from Elizabethkingia anophelis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | H 3 2 |
Unit cell lengths | 102.950, 102.950, 115.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.423 - 1.650 |
R-factor | 0.1464 |
Rwork | 0.145 |
R-free | 0.16350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xat |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX ((1.14_3228)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.423 | 38.423 | 1.690 |
High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
Rmerge | 0.047 | 0.034 | 0.518 |
Rmeas | 0.051 | 0.038 | 0.553 |
Total number of observations | 223684 | ||
Number of reflections | 28379 | 352 | 2063 |
<I/σ(I)> | 21.49 | 41.4 | 3.9 |
Completeness [%] | 99.9 | 98.1 | 100 |
Redundancy | 7.882 | 6.963 | 8.119 |
CC(1/2) | 0.999 | 0.998 | 0.934 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | ElanA.01572.a.B1.PW38419 @21.17 mg/ml was incubated with 5 mM acetyl-CoA and chloramphenicol, then mixed 1:1 with JCSG+(a11): 50% (v/v) MPD, 0.1 M Tris base/ HCl, pH = 8.5, 0.2 M ammonium phosphate basic. Tray 299552a11, puck: jtk9-1 |