6MFH
Mutated Uronate Dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 197.098, 197.098, 197.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.685 - 2.040 |
R-factor | 0.2073 |
Rwork | 0.206 |
R-free | 0.23190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ay3 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.579 |
Data reduction software | xia2 (0.4.0.338) |
Data scaling software | xia2 |
Phasing software | PHASER (1.13) |
Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.700 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.300 | 5.200 |
Rmeas | 0.300 | 5.260 |
Rpim | 0.044 | 0.760 |
Number of reflections | 41593 | 4107 |
<I/σ(I)> | 18 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 47 | |
CC(1/2) | 0.990 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M MES, pH 6.5, 30% MPD |