6MFG
HLA-DQ2-glia-alpha1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.226, 101.968, 70.528 |
| Unit cell angles | 90.00, 91.78, 90.00 |
Refinement procedure
| Resolution | 59.198 - 2.000 |
| R-factor | 0.1877 |
| Rwork | 0.187 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.490 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.044 | 0.393 |
| Number of reflections | 56391 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.999 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 23% PEG3350 and 0.1 M NaH2PO4, pH 6 |
