6MFC
GphF GNAT-like decarboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 138.334, 145.700, 78.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.962 - 2.589 |
R-factor | 0.236 |
Rwork | 0.234 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ree |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.962 | 43.962 | 2.750 |
High resolution limit [Å] | 2.589 | 7.670 | 2.590 |
Rmerge | 0.184 | 0.047 | 2.998 |
Rmeas | 0.192 | 0.049 | 3.114 |
Number of reflections | 24892 | 1046 | 3893 |
<I/σ(I)> | 11.73 | 46.62 | 0.96 |
Completeness [%] | 99.7 | 99.2 | 98.3 |
Redundancy | 13.146 | 12.068 | 13.593 |
CC(1/2) | 0.998 | 0.999 | 0.498 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30-35% PEG 3350, 0.23-0.3 M Ammonium Acetate, 0.1 M BisTris HCl pH 5.5 |