6MFA
Fragment of human fibronectin containing the 4th-7th type III domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-09 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.811, 40.561, 59.217 |
| Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
| Resolution | 33.045 - 1.754 |
| R-factor | 0.1937 |
| Rwork | 0.191 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dft |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.581 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.045 | 1.820 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.100 | 0.660 |
| Number of reflections | 31639 | 2939 |
| <I/σ(I)> | 9.5 | 2.3 |
| Completeness [%] | 95.0 | 89.19 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M MES pH 5.5, 7% PEG 6000 |
