6MF5

Crystal structure of budding yeast Cdc5 polo-box domain in complex with Spc72 phosphopeptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	51.470, 68.190, 86.230
Unit cell angles	90.00, 102.59, 90.00

Refinement procedure

Resolution	42.080 - 2.700
R-factor	0.234
Rwork	0.231
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q4o
RMSD bond length	0.003
RMSD bond angle	0.800
Data reduction software	MOSFLM
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.100	2.780
High resolution limit [Å]	2.700	2.700
Number of reflections	16158	1184
<I/σ(I)>	9.1
Completeness [%]	99.9
Redundancy	5.8
CC(1/2)	0.990	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	100 mM Bis-Tris pH 6.50, 200 mM magnesium chloride, 100 mM cesium chloride, 25 % PEG 3350.