6MF4
Crystal structure of the polo-box domain of Cdc5 from budding yeast.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-29 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.28341 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.080, 65.950, 96.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.060 - 1.800 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q4o |
RMSD bond length | 0.004 |
RMSD bond angle | 0.885 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 30819 | 2611 |
<I/σ(I)> | 12.58 | |
Completeness [%] | 100.0 | |
Redundancy | 4 | |
CC(1/2) | 0.997 | 0.358 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM Bis-Tris pH 8.5, 20 % PEG3350, 200 mM sodium formate. |