6MEQ

PcdhgB3 EC1-4 in 50 mM HEPES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	70
Detector technology	PIXEL
Collection date	2016-12-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	126.811, 162.912, 52.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.919 - 2.900
R-factor	0.2291
Rwork	0.224
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k8r
RMSD bond length	0.001
RMSD bond angle	0.377
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13rc1_2961))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.919	3.004
High resolution limit [Å]	2.900	2.900
Rmerge	0.113
Rmeas	0.125
Number of reflections	11833
<I/σ(I)>	10.02
Completeness [%]	94.2
Redundancy	5.2
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	50 mM HEPES pH7, 10% PEG 5000MME, 4% ethylene glycol