6MEF
Crystal structure of broadly neutralizing antibody AR3C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 76.671, 146.870, 175.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.283 - 2.900 |
R-factor | 0.2231 |
Rwork | 0.221 |
R-free | 0.25610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mwf |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 73.430 | 73.430 | 3.080 |
High resolution limit [Å] | 2.900 | 8.700 | 2.900 |
Rmerge | 0.168 | 0.068 | 1.579 |
Rmeas | 0.185 | 0.076 | 1.729 |
Rpim | 0.076 | 0.033 | 0.695 |
Total number of observations | 129027 | ||
Number of reflections | 22315 | 911 | 3563 |
<I/σ(I)> | 8 | ||
Completeness [%] | 99.8 | 99.5 | 100 |
Redundancy | 5.8 | 5.3 | 6.1 |
CC(1/2) | 0.989 | 0.985 | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | 0.2 M zinc acetate dihydrate pH 6.6, 11 % Sucrose, and 20% PEG 3,350 |