6MEF

Crystal structure of broadly neutralizing antibody AR3C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	76.671, 146.870, 175.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.283 - 2.900
R-factor	0.2231
Rwork	0.221
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mwf
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.430	73.430	3.080
High resolution limit [Å]	2.900	8.700	2.900
Rmerge	0.168	0.068	1.579
Rmeas	0.185	0.076	1.729
Rpim	0.076	0.033	0.695
Total number of observations	129027
Number of reflections	22315	911	3563
<I/σ(I)>	8
Completeness [%]	99.8	99.5	100
Redundancy	5.8	5.3	6.1
CC(1/2)	0.989	0.985	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	298	0.2 M zinc acetate dihydrate pH 6.6, 11 % Sucrose, and 20% PEG 3,350