6MDW
Mechanism of protease dependent DPC repair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 128.422, 29.553, 50.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.580 - 1.500 |
| R-factor | 0.16497 |
| Rwork | 0.163 |
| R-free | 0.19314 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mdx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.401 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rpim | 0.024 | 0.253 |
| Number of reflections | 31618 | 1531 |
| <I/σ(I)> | 30.47 | |
| Completeness [%] | 99.4 | |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M sodium citrate tribasic dihydrate at pH 5.5, 14% (w/v) PEG8000, and 10 mM ATP |
