6MDW

Mechanism of protease dependent DPC repair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	128.422, 29.553, 50.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.580 - 1.500
R-factor	0.16497
Rwork	0.163
R-free	0.19314
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mdx
RMSD bond length	0.008
RMSD bond angle	1.401
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rpim	0.024	0.253
Number of reflections	31618	1531
<I/σ(I)>	30.47
Completeness [%]	99.4
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M sodium citrate tribasic dihydrate at pH 5.5, 14% (w/v) PEG8000, and 10 mM ATP