6MDH

X-ray crystal structure of ISG15 from Myotis davidii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-08
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	I 21 21 21
Unit cell lengths	66.546, 68.426, 72.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.269 - 1.370
R-factor	0.1625
Rwork	0.161
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tla
RMSD bond length	0.014
RMSD bond angle	1.389
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2420: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.390
High resolution limit [Å]	1.370	1.370
Rmeas	0.058	0.641
Number of reflections	34845
<I/σ(I)>	33.8	2.92
Completeness [%]	99.1
Redundancy	9.8
CC(1/2)		0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	citric acid, PEG 6000, Benzamidine hydrochloride