6MDF
Mevalonate kinase from Methanosarcina mazei with 5-phosphomevalonate bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.970, 58.581, 190.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.920 - 2.460 |
R-factor | 0.2038 |
Rwork | 0.200 |
R-free | 0.26720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hac |
RMSD bond length | 0.003 |
RMSD bond angle | 0.510 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.920 | 2.550 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.103 | 0.375 |
Number of reflections | 22260 | 2135 |
<I/σ(I)> | 8.73 | 2.85 |
Completeness [%] | 97.0 | 96.3 |
Redundancy | 4.3 | 4 |
CC(1/2) | 0.999 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100 mM Bis-Tris, pH 5.5, 200-300 mM sodium potassium tartrate, 15-25% PEG8000 |