6MD3
Structure of T. brucei RRP44 PIN domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.2824 |
| Spacegroup name | P 65 |
| Unit cell lengths | 89.570, 89.570, 321.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.910 - 2.290 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.910 | 2.430 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 64571 | 20379 |
| <I/σ(I)> | 13.9 | 1 |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 10.1 | 10 |
| CC(1/2) | 0.990 | 0.420 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291.15 | 20% PEG 3350, 0.2 M magnesium chloride, 0.1 M MES pH 6 |
