6MCJ
Structure of Helical Carotenoid Protein 2 from Fremyella diplosiphon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.162, 71.635, 56.341 |
| Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
| Resolution | 44.263 - 1.712 |
| R-factor | 0.1689 |
| Rwork | 0.167 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1M98 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.678 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.263 | 1.800 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.036 | 0.273 |
| Rmeas | 0.050 | 0.368 |
| Rpim | 0.034 | 0.245 |
| Number of reflections | 59576 | 13974 |
| <I/σ(I)> | 15.7 | 4.1 |
| Completeness [%] | 98.8 | 97.6 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.999 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Ammonium Iodide, 23 % PEG 3.350 |
