6MCA
Structure of Legionella Effector AnkH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-14 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 102.125, 102.125, 265.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.063 - 2.455 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.22370 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.475 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.100 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmeas | 0.082 | 0.855 |
| Number of reflections | 30920 | |
| <I/σ(I)> | 48.1 | 6.1 |
| Completeness [%] | 97.3 | |
| Redundancy | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 1.0 M ammonium tartrate dibasic, pH 7.0 |
