6MC8
Crystal structure of PprA dimer from Deinococcus deserti
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-30 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54056 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.417, 80.985, 148.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.720 - 2.500 |
Rwork | 0.247 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | CrysalisPro (39.29c) |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (1.13-2998) |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.720 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.182 | 0.663 |
Rmeas | 0.189 | 0.700 |
Rpim | 0.049 | 0.223 |
Number of reflections | 22737 | 2079 |
<I/σ(I)> | 19.8 | 2 |
Completeness [%] | 96.7 | 80.2 |
Redundancy | 24.1 | 11.6 |
CC(1/2) | 0.998 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293.15 | Protein at 3.0 mg/mL in 800 mM NaCl, 150 mM Imidazole, 20 mM Tris, pH 8.0 was mixed in 1:1 volume ratio with MCSG3 #92 - a solution of 30 % (w/v) PEG 400 and 0.1 M CHES, pH 9.5. The drop was suspended over 1.5M Ammonium sulfate. |