6MBZ
Structure of Transcription Factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 132.380, 141.400, 72.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.700 - 3.210 |
R-factor | 0.266 |
Rwork | 0.265 |
R-free | 0.28680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y1u |
RMSD bond length | 0.004 |
RMSD bond angle | 0.793 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 70.700 | 70.700 | 3.290 |
High resolution limit [Å] | 3.210 | 14.360 | 3.210 |
Rmerge | 0.105 | 0.032 | 1.152 |
Number of reflections | 23029 | ||
<I/σ(I)> | 13.79 | ||
Completeness [%] | 100.0 | 98.9 | 100 |
Redundancy | 6.6 | 5.5 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 4000 |