6MBW
Structure of Transcription Factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 134.600, 138.620, 76.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.566 - 3.290 |
R-factor | 0.2684 |
Rwork | 0.266 |
R-free | 0.30540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y1u |
RMSD bond length | 0.002 |
RMSD bond angle | 0.626 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 96.570 | 96.570 | 3.380 |
High resolution limit [Å] | 3.290 | 14.710 | 3.290 |
Rmerge | 0.090 | 0.037 | 1.038 |
Number of reflections | 21977 | 2137 | |
<I/σ(I)> | 12.87 | ||
Completeness [%] | 98.5 | 97.6 | 98.4 |
Redundancy | 6.3 | 5.7 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 4000 |