6MB5
AAC-IIIb binary with NEOMYCIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 106.120, 106.120, 68.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.951 - 2.200 |
R-factor | 0.2454 |
Rwork | 0.245 |
R-free | 0.26080 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.6.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.951 | 45.950 | 2.270 |
High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
Rmerge | 0.091 | 0.048 | 0.534 |
Rmeas | 0.107 | 0.057 | 0.634 |
Rpim | 0.055 | 0.029 | 0.334 |
Number of reflections | 22720 | 368 | 1933 |
<I/σ(I)> | 7.5 | ||
Completeness [%] | 98.7 | 98.9 | 97.8 |
Redundancy | 3.5 | 3.5 | 3.3 |
CC(1/2) | 0.995 | 0.995 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 283 | 1-3%PEG 4000, 20-25% isopropanol,0.1M HEPES, pH=7.5 |