6MAN

Crystal structure of a DnaN sliding clamp DNA polymerase III subunit beta from Rickettsia bellii RML369-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	58.880, 81.740, 89.180
Unit cell angles	90.00, 92.87, 90.00

Refinement procedure

Resolution	31.706 - 2.350
R-factor	0.1904
Rwork	0.188
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w7z
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.706	31.706	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.049	0.027	0.596
Rmeas	0.057	0.031	0.681
Number of reflections	35239	391	2580
<I/σ(I)>	16.63	35.67	2.71
Completeness [%]	99.8	91.1	99.9
Redundancy	4.157	3.55	4.21
CC(1/2)	0.999	0.998	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287	RibeA.17987.a.B1.PS38223 at 20 mg/mL against 26% PEG 3350, 0.25 M sodium thiocyanate, supplemented with 20% ethylene glycol as cryo-protectant, crystal tracking ID 301443e9, unique puck ID jka1-3