6MAB

1.7A resolution structure of RsbU from Chlamydia trachomatis (periplasmic domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	I 4
Unit cell lengths	96.712, 96.712, 65.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.073 - 1.700
R-factor	0.1653
Rwork	0.164
R-free	0.19730
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.914
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.360	48.360	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.061	0.032	1.110
Total number of observations	225529
Number of reflections	33233	243	1758
<I/σ(I)>	17.9
Completeness [%]	100.0	99.2	100
Redundancy	6.8	6.7	6.3
CC(1/2)	0.999	0.999	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
2	VAPOR DIFFUSION, SITTING DROP	8.5	298	1 M ammonium phosphate dibasic, 0.1 M Tris
2	VAPOR DIFFUSION, SITTING DROP	8.5	298	1 M ammonium phosphate dibasic, 0.1 M Tris