6MAA

WFIKKN2 Follistatin Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	46.460, 46.460, 95.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.230 - 1.394
R-factor	0.1653
Rwork	0.165
R-free	0.18010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.745
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_2450)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	1.420
High resolution limit [Å]	1.390	1.390
Rmerge	0.058	0.166
Rpim	0.024	0.080
Number of reflections	21677
<I/σ(I)>	25.5
Completeness [%]	99.7
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293.15	ammonium Nitrate, sodium citrate