6M9Z
X-ray Structure of Branchiostoma floridae fluorescent protein lanFP6G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.919, 131.382, 55.076 |
Unit cell angles | 90.00, 116.41, 90.00 |
Refinement procedure
Resolution | 28.000 - 1.200 |
R-factor | 0.128 |
Rwork | 0.128 |
R-free | 0.14600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hvf |
RMSD bond length | 0.012 |
RMSD bond angle | 1.853 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 30.000 | 1.240 |
High resolution limit [Å] | 1.200 | 2.590 | 1.200 |
Rmerge | 0.046 | 0.032 | 0.532 |
Rmeas | 0.053 | 0.037 | 0.630 |
Rpim | 0.027 | 0.019 | 0.334 |
Number of reflections | 153983 | 16154 | 13697 |
<I/σ(I)> | 12.4 | ||
Completeness [%] | 95.3 | 99.1 | 85 |
Redundancy | 3.8 | 3.9 | 3.4 |
CC(1/2) | 0.998 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Na acetate pH 7.0, 12% PEG 3350 |