6M9Y

X-ray Structure of Branchiostoma floridae fluorescent protein lanFP6A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	112.726, 98.169, 55.145
Unit cell angles	90.00, 108.07, 90.00

Refinement procedure

Resolution	28.030 - 1.350
R-factor	0.1333
Rwork	0.133
R-free	0.16880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hvf
RMSD bond length	0.013
RMSD bond angle	1.723
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.400
High resolution limit [Å]	1.350	2.910	1.350
Rmerge	0.044	0.034	0.537
Rmeas	0.053	0.040	0.684
Rpim	0.029	0.021	0.418
Total number of observations	284216
Number of reflections	116582	12072	11220
<I/σ(I)>	10.2
Completeness [%]	93.7	95.9	90.5
Redundancy	2.4	2.8	2.4
CC(1/2)		0.981	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2M Ammonium Citrate pH 7.0, 20% PEG 3350