6M9Y
X-ray Structure of Branchiostoma floridae fluorescent protein lanFP6A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.726, 98.169, 55.145 |
Unit cell angles | 90.00, 108.07, 90.00 |
Refinement procedure
Resolution | 28.030 - 1.350 |
R-factor | 0.1333 |
Rwork | 0.133 |
R-free | 0.16880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hvf |
RMSD bond length | 0.013 |
RMSD bond angle | 1.723 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.400 |
High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
Rmerge | 0.044 | 0.034 | 0.537 |
Rmeas | 0.053 | 0.040 | 0.684 |
Rpim | 0.029 | 0.021 | 0.418 |
Total number of observations | 284216 | ||
Number of reflections | 116582 | 12072 | 11220 |
<I/σ(I)> | 10.2 | ||
Completeness [%] | 93.7 | 95.9 | 90.5 |
Redundancy | 2.4 | 2.8 | 2.4 |
CC(1/2) | 0.981 | 0.731 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M Ammonium Citrate pH 7.0, 20% PEG 3350 |