6M9X
X-ray Structure of Branchiostoma floridae fluorescent protein lanFP10A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1990-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.011, 115.836, 128.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.650 - 1.810 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hvf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.631 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.067 | 0.038 | 0.843 |
| Rmeas | 0.072 | 0.041 | 0.915 |
| Rpim | 0.026 | 0.015 | 0.349 |
| Total number of observations | 579342 | ||
| Number of reflections | 78944 | 8325 | 7733 |
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.6 | 99.9 | 98.8 |
| Redundancy | 7.3 | 7.2 | 6.7 |
| CC(1/2) | 0.999 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Bicine 8.5, 20% PEG 10,000 |
