6M9B
Wild-type streptavidin in complex with biotin solved by native SAD with data collected at 6 keV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1, 2.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.978, 98.322, 52.795 |
| Unit cell angles | 90.00, 112.64, 90.00 |
Refinement procedure
| Resolution | 49.160 - 1.548 |
| R-factor | 0.1551 |
| Rwork | 0.154 |
| R-free | 0.18340 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.368 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 1.604 |
| High resolution limit [Å] | 1.548 | 1.548 |
| Number of reflections | 69231 | |
| <I/σ(I)> | 22.3 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 13.25mg SAV WT + 0.610mg biotin in 500uL H2O in the well: 500uL => 50% MPD sitting drops: 1uL SAV-biot + 1uL 80% MPD, 100mM MMT pH 5.5 |
