6M8H
Crystal Structure of the R208Q mutant of G(i) subunit alpha-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-13 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.558, 79.558, 105.421 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.864 - 2.070 |
| R-factor | 0.1681 |
| Rwork | 0.165 |
| R-free | 0.19730 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.597 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5.620 |
| High resolution limit [Å] | 2.070 | 2.110 |
| Number of reflections | 24084 | |
| <I/σ(I)> | 33.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 297 | 2.0 M (NH4)2SO3 pH 8.0 |
