6M7Z

A divergent kinase lacking the glycine-rich loop regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuole

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.038
Spacegroup name	P 21 21 2
Unit cell lengths	171.466, 123.068, 86.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.700 - 2.500
R-factor	0.1993
Rwork	0.197
R-free	0.23877
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.244
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.088	0.046	0.855
Rmeas	0.095	0.049	0.925
Rpim	0.034	0.018	0.345
Total number of observations	512518
Number of reflections	64181	3431	3158
<I/σ(I)>	9.5
Completeness [%]	99.9	99	99.8
Redundancy	8	7.4	7.3
CC(1/2)		0.997	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	298	0.2M Proline, 0.1M HEPES 7.4, 10% PEG-3350