6M74
Crystal structure of Enterococcus hirae L-lactate oxidase M207L in complex with D-lactate form ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 138.130, 138.130, 127.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.540 - 1.520 |
R-factor | 0.1287 |
Rwork | 0.128 |
R-free | 0.14440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m73 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.387 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.540 | 19.540 | 1.560 |
High resolution limit [Å] | 1.520 | 6.800 | 1.520 |
Rmerge | 0.061 | 0.034 | 0.703 |
Rmeas | 0.063 | 0.035 | 0.731 |
Total number of observations | 1230530 | ||
Number of reflections | 93906 | 1129 | 6875 |
<I/σ(I)> | 26.96 | 66.52 | 4.36 |
Completeness [%] | 99.9 | 94.8 | 99.9 |
Redundancy | 13.104 | 12.805 | 13.078 |
CC(1/2) | 0.999 | 0.999 | 0.942 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Tris, PEG MME |